It varies.
I'm not sure there's an answer 'in general'. Some instructors are glued to their emails, others not. If an instructor is usually pretty good about replying quickly, but isn't for some reason, you might want to see what's going on. If you know your instructor isn't good with emails, go and find them and talk to them in person; instant response.
I also don't quite get what you mean by how long should you give them - before what?
Never assume that because an prof hasn't replied that means you are exempt from following any rules or guides that were posted for the course. That's an easy way to get into hot water. If you need to urgently find out something, use your feet and find somebody to answer it: a peer, a TA, etc.
Andrew Colgoni
Science Fluencies Librarian
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