The waterfront trail is good, start at princess point and goes to bayfront park.
I've ridden on the brantford trail on a road bike, I would recommend it. I've even done the offroad trail a bit there on a road bike, would not recommend that, unless you're crazy.
If you're up for it, try going up Wilson st/County Road 2 to Ancaster. It's all uphill, then coming back downhill is a blast.
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